> For the complete documentation index, see [llms.txt](https://help.bioinsight.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.bioinsight.illumina.com/emedgene/emedgene-analyze-manual/reviewing_a_case/lab_tab/sample-quality-section/ngs-sample-quality-metrics.md).

# NGS sample quality metrics

NGS sample quality metrics provide an overview of QC validation and coverage results for each sample.

{% hint style="info" %}
In v100.41 and later, validations that cannot run reliably are not performed.

* [**Contamination**](/emedgene/emedgene-analyze-manual/reviewing_a_case/lab_tab/sample-quality-section/ngs-sample-quality-metrics/contamination.md) and [**Ploidy**](/emedgene/emedgene-analyze-manual/reviewing_a_case/lab_tab/sample-quality-section/ngs-sample-quality-metrics/ploidy.md) show **N/A** for samples that contain fewer than 10000 variants.
* The [**Pedigree section**](/emedgene/emedgene-analyze-manual/reviewing_a_case/lab_tab/pedigree-section.md) is displayed only for family cases where the [joined VCF file](#user-content-fn-1)[^1] contains at least 10000 variants.
  {% endhint %}

<table data-header-hidden><thead><tr><th width="299.72735595703125">NGS case</th></tr></thead><tbody><tr><td><a href="/emedgene/emedgene-analyze-manual/reviewing_a_case/lab_tab/sample-quality-section/ngs-sample-quality-metrics/ngs-sample-quality.md">Sample quality</a></td></tr><tr><td><a href="/emedgene/emedgene-analyze-manual/reviewing_a_case/lab_tab/sample-quality-section/ngs-sample-quality-metrics/ngs-sex-validation.md">Sex validation</a></td></tr><tr><td><a href="/emedgene/emedgene-analyze-manual/reviewing_a_case/lab_tab/sample-quality-section/ngs-sample-quality-metrics/ploidy.md">Ploidy</a>*</td></tr><tr><td><a href="/emedgene/emedgene-analyze-manual/reviewing_a_case/lab_tab/sample-quality-section/ngs-sample-quality-metrics/contamination.md">Contamination</a></td></tr><tr><td><p><a href="/emedgene/emedgene-analyze-manual/reviewing_a_case/lab_tab/sample-quality-section/ngs-sample-quality-metrics/coverage.md">Coverage metrics</a>:</p><ul><li>Average coverage</li><li>% Bases with coverage >10x</li><li>% Bases with coverage >20x</li></ul></td></tr><tr><td><a href="/emedgene/emedgene-analyze-manual/reviewing_a_case/lab_tab/sample-quality-section/ngs-sample-quality-metrics/percentage-of-mapped-reads.md">% Mapped reads</a></td></tr><tr><td><a href="/emedgene/emedgene-analyze-manual/reviewing_a_case/lab_tab/sample-quality-section/ngs-sample-quality-metrics/sequencing-error-rate.md">Error rate</a></td></tr></tbody></table>

\*<sub>*Available only for whole genome FASTQ cases.*</sub>

[^1]: A Variant Call Format file that merges the proband's variants with family members' variants.


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