> For the complete documentation index, see [llms.txt](https://help.bioinsight.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.bioinsight.illumina.com/dragen-protein-quantification/counting-and-normalization/metrics-appendix.md).

# Metrics Appendix

## Sample Level Metrics

Sample level metrics are found in the sample/row-level metadata in the ADAT.

<table><thead><tr><th width="136">Metric</th><th>ADAT Field</th><th>Applied To</th><th>Applies To (Kit Type)</th><th>Threshold</th><th>Impact to Sample</th></tr></thead><tbody><tr><td>Minimum SOMAmer Read Count</td><td>SOMAmerReads; SOMAmerReads_PassFlag</td><td>Non-Blank Samples</td><td>Plasma, Serum, CSF</td><td>SOMAmerReads > 10 million</td><td>PASS/FLAG</td></tr><tr><td>Maximum SOMAmer Normalized Read Count</td><td>SOMAmerNormReads; SOMAmerNormReads_PassFlag</td><td>Blank Samples</td><td>All kits</td><td>SOMAmerNormReads &#x3C; 20 million reads</td><td>PASS/FLAG</td></tr><tr><td>Hybridization QC</td><td>HybQC; HybQC_PassFlag; HybQC_Ref (header); HybQC_Delta (header)</td><td>Non-Blank Samples</td><td>All kits</td><td>HybQC ≤ HybQC_Delta</td><td>PASS/FLAG</td></tr><tr><td>Hybridization Normalization</td><td>HybNorm_1_ScaleFactor; HybNorm_PassFlag</td><td>Non-Blank Samples</td><td>Plasma, Serum</td><td>0.4 ≤ HybNorm_1_ScaleFactor ≤ 2.5</td><td>PASS/FLAG</td></tr><tr><td>Hybridization Normalization</td><td>HybNorm_1_ScaleFactor; HybNorm_PassFlag</td><td>Calibrator and QC Samples only</td><td>MultiMatrix</td><td>0.4 ≤ HybNorm_1_ScaleFactor ≤ 2.5</td><td>PASS/FLAG</td></tr><tr><td>Hybridization Normalization</td><td>HybNorm_1_ScaleFactor; HybNorm_PassFlag</td><td>Calibrator and QC Samples</td><td>CSF</td><td>0.4 ≤ HybNorm_1_ScaleFactor ≤ 2.5</td><td>PASS/FLAG</td></tr><tr><td>Hybridization Normalization</td><td>HybNorm_1_ScaleFactor; HybNorm_PassFlag</td><td>Customer Samples</td><td>CSF</td><td>0.2 ≤ HybNorm_1_ScaleFactor ≤ 5.0</td><td>PASS/FLAG</td></tr><tr><td>Internal Reference Median Normalization</td><td>MedNormInt_&#x3C;Dilution>_ScaleFactor; MedNormInt_PassFlag</td><td>Individual Blank and Calibrator samples, split by dilution group</td><td>All kits</td><td>0.4 ≤ MedNormInt_&#x3C;Dilution>_ScaleFactor ≤ 2.5</td><td>PASS/FLAG</td></tr><tr><td>External Reference Median Normalization</td><td>MedNormExt_&#x3C;Dilution>_ScaleFactor; MedNormExt_PassFlag</td><td>Individual non-control and QC samples, split by dilution group</td><td>Plasma, Serum, CSF</td><td>0.4 ≤ SF ≤ 2.5 (Plasma, Serum, CSF QC); 0.125 ≤ SF ≤ 8.0 (CSF samples)</td><td>PASS/FLAG</td></tr><tr><td>Cross-Plate Internal Median Normalization</td><td>CrossPlateMedNormInt_ScaleFactor; CrossPlateMedNormInt_PassFlag</td><td>MultiMatrix Samples</td><td>MultiMatrix</td><td>0.4 ≤ CrossPlateMedNormInt_ScaleFactor ≤ 2.5</td><td>PASS/FLAG</td></tr><tr><td>Row Check</td><td>RowCheck_PassFlag</td><td>All samples</td><td>All kits</td><td>PLATE_FAIL if the sample is on a plate that received a FAIL. Otherwise, PASS if all Pass Flags in this row are PASS and FLAG if not.</td><td>PASS/FLAG/PLATE_FAIL</td></tr></tbody></table>

> **MultiMatrix kit notes:**
>
> * MultiMatrix does not use External Reference Median Normalization (uses Cross-Plate Internal Median Normalization instead)
> * MultiMatrix uses PlatformSpecificQCCheckTailPercent (at plate level) instead of PlatformSpecificCalibrateTailPercent

## SOMAmer Level Metrics

SOMAmer level metrics are found in the SOMAmer/column-level metadata in the ADAT.

<table><thead><tr><th width="290.765625">Metric</th><th>ADAT Field</th><th>Applies To (Kit Type)</th></tr></thead><tbody><tr><td>Platform Specific Calibration</td><td>PlatformSpecificCalibrate_&#x3C;PlateBarcode>_ScaleFactor</td><td>Plasma, Serum, CSF</td></tr><tr><td>Cross Platform Calibration</td><td>CrossPlatformCalibrate_&#x3C;PlateBarcode>_ScaleFactor</td><td>Plasma, Serum, CSF</td></tr><tr><td>QC Check</td><td>QCCheck_&#x3C;PlateBarcode>_ScaleFactor</td><td>Plasma, Serum, CSF</td></tr><tr><td>Platform Specific QC Check</td><td>PlatformSpecificQCCheck_&#x3C;PlateBarcode>_ScaleFactor</td><td>MultiMatrix</td></tr></tbody></table>

## Plate Level Metrics

Plate level metrics are found in the header metadata in the ADAT.

| Metric                                  | ADAT Field                                                                           | Applies To (Kit Type)                                | Threshold                                          | Impact to Plate |
| --------------------------------------- | ------------------------------------------------------------------------------------ | ---------------------------------------------------- | -------------------------------------------------- | --------------- |
| QC Percent in Tails                     | QCCheckTailPercent; QCCheckTailPercent\_PassFlag                                     | Plasma, Serum, CSF                                   | QCCheckTailPercent < 15%                           | PASS/FAIL       |
| Calibration Percent in Tails            | PlatformSpecificCalibrateTailPercent; PlatformSpecificCalibrateTailPercent\_PassFlag | Plasma, Serum, CSF                                   | PlatformSpecificCalibrateTailPercent < 15%         | PASS/WARNING    |
| QC Check Percent in Tails (MultiMatrix) | PlatformSpecificQCCheckTailPercent; PlatformSpecificQCCheckTailPercent\_PassFlag     | MultiMatrix                                          | PlatformSpecificQCCheckTailPercent < 15%           | PASS/WARNING    |
| SOMAmer Normalized Reads                | PlateSOMAmerNormReads\_PassFlag                                                      | All kits                                             | < 70% of blank samples flagged at SOMAmerNormReads | PASS/WARNING    |
| Platform Specific Plate Scale           | PlatformSpecificPlateScale\_ScaleFactor                                              | All kits                                             | N/A                                                | N/A             |
| Cross Platform Plate Scale              | CrossPlatformPlateScale\_ScaleFactor                                                 | All kits                                             | N/A                                                | N/A             |
| Cross Platform Calibration              | CrossPlatformCalibrationTailPercent                                                  | Plasma, Serum, CSF                                   | N/A                                                | N/A             |
| IntraPlateMedianCV                      | IntraPlateMedianCV                                                                   | All kits (Plasma/Serum/CSF: Cal+QC; MultiMatrix: QC) | N/A                                                | Informational   |
| InterPlateMedianCV                      | InterPlateMedianCV                                                                   | Plasma, Serum, CSF (requires ≥4 plates)              | N/A                                                | Informational   |
| MedianS2B                               | MedianS2B                                                                            | All kits (Plasma/Serum/CSF: Cal+QC; MultiMatrix: QC) | N/A                                                | Informational   |


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