> For the complete documentation index, see [llms.txt](https://help.bioinsight.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.bioinsight.illumina.com/dragen-mirna/readme/reference-database.md).

# Reference Database

DRAGEN miRNA app offers users the option to select from two reference databases. This section outlines the process of constructing these databases.

### Reference v21

Mature miRNA and miRNA hairpin libraries were obtained from [miRBase.org](https://www.mirbase.org/) using version v21. Human (*Homo sapiens*), mouse (*Mus musculus*), rat (*Rattus norvegicus*), fruitfly (*Drosophila melanogaster*), nematode (*Caenorhabditis elegans*) and zebrafish (*Danio rerio*) mRNA and other noncoding RNA libraries were obtained from Ensembl ([www.ensembl.org/](http://www.ensembl.org/)), unless otherwise denoted. Human tRNAs were obtained from the Genomic [tRNA Database](https://pubmed.ncbi.nlm.nih.gov/18984615/). Human snoRNA was obtained from the [snoRNABase](https://www-snorna.biotoul.fr/).

### Reference v22

The libraries used when selecting reference v22 are available here [SourceForge](https://sourceforge.net/projects/mirge3/files/miRge3_Lib/). Mature miRNA and miRNA hairpin libraries were obtained from [miRBase.org](https://www.mirbase.org/) using version v22.

### Available Species

List of species supported in the software (compatible with both v21 and v22):

* Human (*Homo sapiens*)
* Mouse (*Mus musculus*)
* Rat (*Rattus norvegicus*)
* Fruitfly (*Drosophila melanogaster*)
* Nematode (*Caenorhabditis elegans*)
* Zebrafish (*Danio rerio*)


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